Journal: Nature Metabolism

Article Title: The source of dietary fat influences anti-tumour immunity in obese mice

doi: 10.1038/s42255-025-01330-w

Figure Lengend Snippet: a-f , NK cells were isolated from the spleens of naïve mice fed HFD derived from Butter, Palm Oil, or SFD for 12 weeks and stimulated with IL-2, IL-12, and IL-15 for 20 hr. ( a ) Relative Ifng gene expression. ( b ) Heatmap of 50 most differentially expressed proteins detected by mass spectrometry. Abundance of NKG2D ( c ), ARL8B ( d ), STAT5B ( e ), and mTOR ( f ) detected by mass spectrometry. g, h , Representative histogram ( g , left) and summary data (g, right) of LipidTOX MFI of circulating CD8 T cells from mice fed SFD (gray), butter-HFD (pink), or palm oil-HFD (blue). ( a ) n = 2; ( b - f ) SFD n = 4, Butter n = 4, Palm Oil n = 5; ( g ) n = 5. In bar graphs, bar height represents mean with error bars ± SEM. Comparisons were made using one-way ANOVA followed by Sidak’s multiple comparisons test.

Article Snippet: For analysis of total lipid levels, cells were stained with LipidTOX Green Neutral Lipid Stain (Fisher Scientific, diluted 1:1,000) in PBS for 30 min at 4 °C.

Techniques: Isolation, Derivative Assay, Gene Expression, Mass Spectrometry

Journal: Nature Metabolism

Article Title: The source of dietary fat influences anti-tumour immunity in obese mice

doi: 10.1038/s42255-025-01330-w

Figure Lengend Snippet: a , b , Representative histograms ( a ) and summary data ( b ) of LipidTOX in NK cells from mice fed HFD derived from butter (pink), palm oil (blue) or SFD (grey). MFI, mean fluorescence intensity. c – h , Seahorse real-time metabolic flux assays of splenic NK cells from mice fed SFD, butter or palm oil for 12 weeks and stimulated with IL-2, IL-12 and IL-15. Extracellular acidification rate (ECAR) ( c ) of purified NK cells was measured to assess basal glycolysis ( d ) and glycolytic capacity ( e ). Oxygen consumption rate (OCR) ( f ) of purified NK cells was measured to determine basal oxidative phosphorylation ( g ) and maximal respiratory rate ( h ). i – n , Proteomic analysis of splenic NK cells stimulated with IL-2, IL-12 and IL-15: volcano plot representing proteins differentially expressed between mice fed butter-HFD and palm oil-HFD ( i ); KEGG pathway analysis of proteins upregulated in NK cells from mice fed palm oil relative to mice fed butter ( j ); expression of NK cell activation-related proteins NKp46 ( k ), NK1.1 ( l ) and NEMO ( m ) and ChIP-X enrichment analysis (ChEA) of proteins significantly increased in NK cells from mice fed palm oil relative to those fed butter ( n ). o , Western blot analysis of c-Myc expression in NK cells pooled from five mice per diet. p , c-Myc expression in splenic NK cells stimulated with IL-2, IL-12 and IL-15 for 4 h, measured by flow cytometry. Sample sizes are as follows: in a and b , n = 7, data combined from two independent experiments; in c and f , n = 3, representative of three independent experiments; in d and e , SFD n = 11, butter n = 10, palm oil n = 11, combined from three independent experiments; in g and h , SFD n = 10, butter n = 9, palm oil n = 11, combined from three independent experiments; in i – n , SFD n = 4, butter n = 4, palm oil n = 5; in p , SFD n = 7, butter n = 6, palm oil n = 8. In bar graphs, bar height represents the mean; error bars, s.e.m. In line graphs, each data point represents the mean; error bars, s.e.m. Comparisons were made using one-way ANOVA followed by Tukey’s multiple comparisons test. Oligo, oligomycin; 2DG, 2-deoxyglucose; Rot, rotenone plus antimycin-A.

Article Snippet: For analysis of total lipid levels, cells were stained with LipidTOX Green Neutral Lipid Stain (Fisher Scientific, diluted 1:1,000) in PBS for 30 min at 4 °C.

Techniques: Derivative Assay, Fluorescence, Purification, Phospho-proteomics, Expressing, Activation Assay, Western Blot, Flow Cytometry

Journal: International Journal of Pharmaceutics: X

Article Title: Allicin-based biomimetic nanoparticles of the erythrocyte membrane for the delivery of lumefantrine to enhance its antimalarial effect

doi: 10.1016/j.ijpx.2026.100487

Figure Lengend Snippet: Neutralization of merozoites. A Purification of infected erythrocytes using the Percoll separation solution, displaying infected (black arrow) and normal (red arrow) erythrocytes. B Confocal fluorescent images of merozoites (blue), PECm-Allicin@LM (red), and their colocalization (purple). Scale bar = 50 μm. C Representative scatter plots of Hoechst 33342/CFDA-SE for the invasion test of merozoites and normal erythrocytes after drug treatment. D Intrusion rate (the percentage of cells in the Q2 area in each group relative to that in the Q2 area in the Model group). Data are presented as mean ± SEM ( n = 3), * P < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The red fluorescent cell membrane dye DiD was purchased from Shanghai Titan Scientific Co., Ltd. (China).

Techniques: Neutralization, Purification, Infection